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KMID : 1094720140190010043
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Biotechnology and Bioprocess Engineering
2014 Volume.19 No. 1 p.43 ~ p.51
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Pretreatment with recombinant human interleukin 2 (rhIL-2) Up-regulates PCNA-positive cells after partial hepatectomy in rat liver
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Jeon Mi-Ae
Kwon Hyun-Jung
Kim Yong-Hyun
Han Kook-Il
Nam Kung-Woo
Baik Yeong-Jun
Lee Sung-Hee
Kim Wan-Jong
Han Man-Deuk
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Abstract
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We prepared recombinant human interleukin-2 (rhIL-2) and studied its pretreated influence on liver regeneration and the blood profile in partially (67%) hepatectomized (PH) male Sprague-Dawley rats. Rats were injected in the tail vein with rhIL-2 three times per day for 3 consecutive days and 67% underwent a partial hepatectomy (PH). Five days after the PH, liver tissue and blood samples were analyzed for liver regeneration and hematological changes. The weight of the liver in the rhIL-2 pretreated groups increased in a dose-dependent manner; with the highest treatment (24 × 104 IU/kg), the maximum liver weight of 88.6% was exhibited. The control group showed a gradual increase to 76.3% of the original liver weight. A histological analysis of the liver showed an increase in proliferating cell nuclear antigen (PCNA)-positive cells in rhIL-2 pretreated rat livers. The rate of hepatocyte proliferation also increased significantly in primary cultured rat liver cells following rhIL-2 treatment. These results suggest that pretreatment with rhIL-2 may play adjuvant roles in liver regeneration after PH.
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KEYWORD
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rhIL-2, partial hepatectomy (PH), PCNA, immunohistochemistry, liver regeneration
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